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CoraLite® Plus 405 Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

MOPC-21

Cat no : CL405-65124

Print datasheet

Synonyms

IgG1, IgG1 Isotype Control, mouse IgG1, Mouse IgG1 Isotype Control

验证数据展示

  • FC experiment of rat splenocytes using CL405-65124

    FC experiment of rat splenocytes using CL405-65124

    1X10^6 rat splenocytes cells were surface stained with 0.5 ug CoraLite® Plus 405 Mouse IgG1 Isotype Control (CL405-65124, Clone:MOPC-21) (red), or 0.5 ug CoraLite® Plus 405 Anti-Rat CD45 (green) or unstained (blue). Cells were not fixed.

经过测试的应用

Positive FC detected in
For other applications, we recommend the unconjugated version of this antibody, 65124-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

Tested Applications FC Application Description
Tested Reactivity
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant gamma 1 (G1m marker)
Synonyms IgG1, IgG1 Isotype Control, mouse IgG1, Mouse IgG1 Isotype Control
Gene Symbol IgG1 Isotype Control
Gene ID (NCBI) 16017
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission Maxima Wavelengths399 nm / 422 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01868
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 IgG1 Isotype Control antibody CL405-65124Download protocol
Standard Protocols
Click here to view our Standard Protocols