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CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

MOPC-21

Cat no : CL647-65124

Print datasheet

Synonyms

CoraLite®647-conjugated Mouse IgG1 Isotype Control, IgG1, IgG1 Isotype Control, mouse IgG1

验证数据展示

  • FC experiment of human PBMCs using CL647-65124

    FC experiment of human PBMCs using CL647-65124

    1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21) (red), CL647 Anti-Human CD3 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC (Intra) detected in
Positive FC detected in
For other applications, we recommend the unconjugated version of this antibody, 65124-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

Tested Applications FC Application Description
Tested Reactivity
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant gamma 1 (G1m marker)
Synonyms CoraLite®647-conjugated Mouse IgG1 Isotype Control, IgG1, IgG1 Isotype Control, mouse IgG1
Gene Symbol IgG1 Isotype Control
Gene ID (NCBI) 16017
RRIDAB_2920260
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01868
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IgG1 Isotype Control antibody CL647-65124Download protocol
Standard Protocols
Click here to view our Standard Protocols