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  • Featured Product
  • KD/KO Validated

JAK1 Monoclonal antibody, PBS Only

JAK1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3H7A8

Cat no : 66466-1-PBS

Print datasheet

Synonyms

JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1

验证数据展示

  • WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

    WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

    IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with JAK1 antibody included
  • IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

    IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with JAK1 antibody included
Planning an IHC experiment? We recommend our IHCeasy JAK1 Ready-To-Use IHC Kit. JAK1 primary antibody included.

产品信息

66466-1-PBS targets JAK1 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat, mouse
Immunogen JAK1 fusion protein Ag17940 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Janus kinase 1 (a protein tyrosine kinase)
Synonyms JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1
Calculated Molecular Weight 1154 aa, 133 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberBC132729
Gene Symbol JAK1
Gene ID (NCBI) 3716
RRIDAB_2881834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23458
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.