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验证数据展示

  • WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

    WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

    WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)

    FC experiment of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human JNK (66210-1-Ig, Clone:1A12E1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

    IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAPK8/JNK antibody included
  • IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

    IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAPK8/JNK antibody included
  • IF Staining of Jurkat using 66210-1-Ig (same clone as 66210-1-PBS)

    IF Staining of Jurkat using 66210-1-Ig (same clone as 66210-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (66210-1-Ig, Clone: 1A12E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

JNK Monoclonal antibody, PBS Only

JNK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66210-1-PBS

产品说明书

CloneNo. 1A12E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

MAPK8, JNK1, JNK 2, JNK 1, EC:2.7.11.24

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.

产品信息

66210-1-PBS targets JNK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 JNK fusion protein Ag21426 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 8
别名 MAPK8, JNK1, JNK 2, JNK 1, EC:2.7.11.24
计算分子量 48 kDa
观测分子量 44-48 kDa, 50-55 kDa
GenBank蛋白编号BC130572
基因名称 JNK
Gene ID (NCBI) 5599
RRIDAB_2881601
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP45983
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.