验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, rat liver tissue, HepG2 cells, Jurkat cells, HeLa cells, mouse liver tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10827-1-AP targets TIP60/KAT5 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | TIP60/KAT5 fusion protein Ag1261 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | K(lysine) acetyltransferase 5 |
| 别名 | 60 kDa Tat interactive protein, cPLA(2) interacting protein, cPLA2, ESA1, Histone acetyltransferase KAT5, HIV 1 Tat interactive protein, HTATIP, HTATIP1, K(lysine) acetyltransferase 5, KAT5, Lysine acetyltransferase 5, PLIP, TIP, TIP60 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 58 kDa, 70 kDa |
| GenBank蛋白编号 | BC000166 |
| 基因名称 | KAT5 |
| Gene ID (NCBI) | 10524 |
| RRID | AB_2128431 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92993 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis. KAT5 exists some isoforms with MV 53-60 kDa
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TIP60/KAT5 antibody 10827-1-AP | Download protocol |
| IHC protocol for TIP60/KAT5 antibody 10827-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells. | ||
Nat Commun Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress.
| ||
Cancer Lett Acetylation-dependent deubiquitinase USP26 stabilizes BAG3 to promote breast cancer progression | ||
J Mol Cell Biol Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis |
