验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, mouse brain tissue, rat brain tissue, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy KATNA1 Ready-To-Use IHC Kit. KATNA1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17560-1-AP targets KATNA1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | KATNA1 fusion protein Ag11753 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | katanin p60 (ATPase-containing) subunit A 1 |
| 别名 | p60 katanin, Katanin p60 subunit A1, Katanin p60 ATPase-containing subunit A1, EC:5.6.1.1 |
| 计算分子量 | 55 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC050428 |
| 基因名称 | KATNA1 |
| Gene ID (NCBI) | 11104 |
| RRID | AB_10694670 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75449 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KATNA1 antibody 17560-1-AP | Download protocol |
| IHC protocol for KATNA1 antibody 17560-1-AP | Download protocol |
| IP protocol for KATNA1 antibody 17560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly. | ||
Nat Cell Biol Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex. | ||
Neuron Katanin p80 regulates human cortical development by limiting centriole and cilia number. | ||
PLoS Biol Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding. | ||
Elife Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission |
