验证数据展示
经过测试的应用
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24421-1-AP targets TALPID3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP, IF |
| 经测试反应性 | human, mouse, canine |
| 文献引用反应性 | human, mouse, caenorhabditis elegans |
| 免疫原 | TALPID3 fusion protein Ag19813 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0586 |
| 别名 | KIAA0586, KIAA0586 isoform, Talpid3 |
| 计算分子量 | 1533 aa, 169 kDa |
| GenBank蛋白编号 | BC066647 |
| 基因名称 | TALPID3 |
| Gene ID (NCBI) | 9786 |
| RRID | AB_2879539 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BVV6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIAA0586 also named as Talpid3 is a 1472 amino-acid protein, which belongs to Talpid3 family. Talpid3 is a gene essential for vertebrate development. Studies in chicken, mouse, zebrafish and human have shown that TALPID3 is a centrosomal protein, mutants null for TALPID3 fail to produce cilia, resulting in far reaching signalling defects such as polydactyly, dorsalised neural tube, craniofacial and vascular defects often due to abnormalities in Hedgehog signal transduction. Talpid3 is absolutely required for the function of both Gli repressor and activator in the intracellular Hedgehog pathway and ciliogenesis. TALPID3 may be identified as a subcellular maker of centrosome and needed to further studied.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TALPID3 antibody 24421-1-AP | Download protocol |
| IF protocol for TALPID3 antibody 24421-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.
| ||
Elife TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23). | ||
EMBO Rep Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function. | ||
Development Centrosomal protein CP110 controls maturation of mother centriole during cilia biogenesis. |