验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, BGC-823 cells, HeLa cells, human brain tissue, mouse cerebellum tissue, mouse testis tissue, mouse thymus tissue, Jurkat cells, HEK-293 cells, MCF-7 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse heart tissue, mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18258-1-AP targets ATG13 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ATG13 fusion protein Ag13090 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0652 |
| 别名 | ATG13, Autophagy related protein 13, KIAA0652 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 57-63 kDa |
| GenBank蛋白编号 | BC001331 |
| 基因名称 | ATG13 |
| Gene ID (NCBI) | 9776 |
| RRID | AB_2130658 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75143 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG13 antibody 18258-1-AP | Download protocol |
| IHC protocol for ATG13 antibody 18258-1-AP | Download protocol |
| IF protocol for ATG13 antibody 18258-1-AP | Download protocol |
| IP protocol for ATG13 antibody 18258-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest p53/microRNA-214/ULK1 axis impairs renal tubular autophagy in diabetic kidney disease. | ||
Autophagy The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy. | ||
Oxid Med Cell Longev Silencing TUFM Inhibits Development of Monocrotaline-Induced Pulmonary Hypertension by Regulating Mitochondrial Autophagy via AMPK/mTOR Signal Pathway | ||
Stem Cell Res Ther Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis. | ||
Ecotoxicol Environ Saf Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain. |
