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验证数据展示

  • WB analysis using 67843-1-Ig

    WB analysis using 67843-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67843-1-Ig

    WB analysis of HSC-T6 using 67843-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67843-1-Ig

    IF Staining of HeLa using 67843-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KIAA1524 antibody (67843-1-Ig, Clone: 2A3F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CIP2A Monoclonal antibody

CIP2A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67843-1-Ig

产品说明书

CloneNo. 2A3F3

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

WB, IF/ICC, ELISA

别名

Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, p90, p90 autoantigen, Protein CIP2A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, PC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67843-1-Ig targets CIP2A in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, rat
免疫原 CIP2A fusion protein Ag19598 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA1524
别名 Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, p90, p90 autoantigen, Protein CIP2A
计算分子量 905 aa, 102 kDa
观测分子量 102 kDa
GenBank蛋白编号BC130564
基因名称 CIP2A
Gene ID (NCBI) 57650
RRIDAB_2918605
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TCG1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)

实验方案

Product Specific Protocols
WB protocol for CIP2A antibody 67843-1-IgDownload protocol
IF protocol for CIP2A antibody 67843-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols