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验证数据展示

  • IF Staining of HeLa using CL594-67843

    IF Staining of HeLa using CL594-67843

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 CIP2A antibody (CL594-67843, Clone: 2A3F3 ) at dilution of 1:200.

CoraLite®594-conjugated CIP2A Monoclonal antibody

CIP2A Monoclonal Antibody for IF/ICC
Cat No. CL594-67843

产品说明书

CloneNo. 2A3F3

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

IF/ICC

别名

Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, p90, p90 autoantigen, Protein CIP2A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67843-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67843 targets CIP2A in IF/ICC applications and shows reactivity with Human, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, rat
免疫原 CIP2A fusion protein Ag19598 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA1524
别名 Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, p90, p90 autoantigen, Protein CIP2A
计算分子量 905 aa, 102 kDa
观测分子量 102 kDa
GenBank蛋白编号BC130564
基因名称 CIP2A
Gene ID (NCBI) 57650
RRIDAB_2934781
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TCG1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)

实验方案

Product Specific Protocols
IF protocol for CL594 CIP2A antibody CL594-67843Download protocol
Standard Protocols
Click here to view our Standard Protocols