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验证数据展示

  • FC experiment of HeLa using CL488-67843

    FC experiment of HeLa using CL488-67843

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CIP2A (CL488-67843, Clone:2A3F3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67843

    IF Staining of HeLa using CL488-67843

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CIP2A antibody (CL488-67843, Clone: 2A3F3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CIP2A Monoclonal antibody

CIP2A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67843

产品说明书

CloneNo. 2A3F3

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

IF/ICC, FC (Intra)

别名

KIAA1524, Protein CIP2A, p90 autoantigen, p90, Cancerous inhibitor of PP2A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67843-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67843 targets CIP2A in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat
免疫原 CIP2A fusion protein Ag19598 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA1524
别名 KIAA1524, Protein CIP2A, p90 autoantigen, p90, Cancerous inhibitor of PP2A
计算分子量 905 aa, 102 kDa
观测分子量 102 kDa
GenBank蛋白编号BC130564
基因名称 CIP2A
Gene ID (NCBI) 57650
RRIDAB_2923799
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TCG1
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CIP2A antibody CL488-67843Download protocol
FC protocol for CL Plus 488 CIP2A antibody CL488-67843Download protocol
Standard Protocols
Click here to view our Standard Protocols