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验证数据展示

  • WB analysis of HeLa using 14232-1-AP

    WB analysis of HeLa using 14232-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14232-1-AP (Kallikrein 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 14232-1-AP

    IHC staining of human ovary cancer using 14232-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14232-1-AP (Kallikrein 8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK8 antibody included

Kallikrein 8 Polyclonal antibody

Kallikrein 8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14232-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KLK8, KLK 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KLK8 Ready-To-Use IHC Kit. KLK8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14232-1-AP targets Kallikrein 8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Kallikrein 8 fusion protein Ag5472 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kallikrein-related peptidase 8
别名 KLK8, KLK 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8
计算分子量 28 kDa
观测分子量 30 kDa
GenBank蛋白编号BC040887
基因名称 KLK8
Gene ID (NCBI) 11202
RRIDAB_2134643
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60259
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Kallikrein 8 antibody 14232-1-APDownload protocol
IHC protocol for Kallikrein 8 antibody 14232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Authors - Tongmei Zhang
View Article