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  • Featured Product
  • KD/KO Validated

KRAS Polyclonal antibody, PBS Only

KRAS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 12063-1-PBS

Print datasheet

Synonyms

KRAS4A, KRAS4B, pan RAS, GTPase KRas, EC:3.6.5.2

验证数据展示

  • WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    WB analysis using 12063-1-AP (same clone as 12063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 12063-1-AP (same clone as 12063-1-PBS)

    WB analysis of HEK-293 using 12063-1-AP (same clone as 12063-1-PBS)

    WB result of KRAS antibody (12063-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRAS transfected HEK-293 cells. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    IHC staining of human lung cancer using 12063-1-AP (same clone as 12063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    IF Staining of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRAS antibody (12063-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    FC experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KRAS (12063-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    IF Staining of mouse brain using 12063-1-AP (same clone as 12063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KRAS antibody (12063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    IP experiment of HeLa using 12063-1-AP (same clone as 12063-1-PBS)

    IP result of anti-KRAS (IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:10000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 12063-1-PBS in a different storage buffer formulation.

产品信息

12063-1-PBS targets KRAS in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KRAS fusion protein Ag2700 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Synonyms KRAS4A, KRAS4B, pan RAS, GTPase KRas, EC:3.6.5.2
Calculated Molecular Weight 188 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC013572
Gene Symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01116
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.