• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

KU70,XRCC6 Monoclonal antibody, PBS Only

KU70,XRCC6 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

3C12D9

Cat No : 66607-1-PBS

Print datasheet

Synonyms

5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, KU70,XRCC6, ML8, Thyroid lupus autoantigen, TLAA, XRCC6

验证数据展示

  • WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    IF Staining of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66607-1-Ig (same clone as 66607-1-PBS)

    IF Staining of HeLa using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    FC experiment of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human KU70,XRCC6 (66607-1-Ig, Clone:3C12D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XRCC6 antibody included
Planning an IHC experiment? We recommend our IHCeasy XRCC6 Ready-To-Use IHC Kit. XRCC6 primary antibody included.

产品信息

66607-1-PBS targets KU70,XRCC6 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity Human, mouse, rat
Immunogen KU70,XRCC6 fusion protein Ag21301 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Synonyms 5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, KU70,XRCC6, ML8, Thyroid lupus autoantigen, TLAA, XRCC6
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC008343
Gene Symbol XRCC6
Gene ID (NCBI) 2547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12956
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.