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验证数据展示

  • WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    WB analysis using 66607-1-Ig (same clone as 66607-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    IF Staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    IF Staining of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66607-1-Ig (same clone as 66607-1-PBS)

    IF Staining of HeLa using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KU70,XRCC6 antibody (66607-1-Ig, Clone: 3C12D9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    FC experiment of HepG2 using 66607-1-Ig (same clone as 66607-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human KU70,XRCC6 (66607-1-Ig, Clone:3C12D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    IHC staining of human breast cancer using 66607-1-Ig (same clone as 66607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66607-1-Ig (KU70,XRCC6 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66607-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XRCC6 antibody included

KU70,XRCC6 Monoclonal antibody, PBS Only

KU70,XRCC6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66607-1-PBS

产品说明书

CloneNo. 3C12D9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

KU70, XRCC6, 3C12D9, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy XRCC6 Ready-To-Use IHC Kit. XRCC6 primary antibody included.

产品信息

66607-1-PBS targets KU70,XRCC6 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KU70,XRCC6 fusion protein Ag21301 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 X-ray repair complementing defective repair in Chinese hamster cells 6
别名 KU70, XRCC6, 3C12D9, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70
计算分子量 75 kDa
观测分子量 70 kDa
GenBank蛋白编号BC008343
基因名称 KU70
Gene ID (NCBI) 2547
RRIDAB_2881967
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12956
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.