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Ki-67 Recombinant antibody

Ki-67 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241499E7

Cat no : 84192-4-RR

Print datasheet

Synonyms

MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67

验证数据展示

  • IHC staining of human tonsillitis using 84192-4-RR

    IHC staining of human tonsillitis using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KI67 antibody included
  • IHC staining of human tonsillitis using 84192-4-RR

    IHC staining of human tonsillitis using 84192-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KI67 antibody included
  • IF Staining of human lung cancer using 84192-4-RR

    IF Staining of human lung cancer using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84192-4-RR

    IF Staining of HeLa using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of human lung cancer using 84192-4-RR

    IF Staining of human lung cancer using 84192-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 84192-4-RR

    WB analysis using 84192-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84192-4-RR (Ki-67 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, MCF-7 cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human Breast cancer tissue, human malignant melanoma tissue, human prostate cancer tissue, Human renal cancer, human skin cancer tissue, Human Spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue, human thyroid cancer tissue
Positive IF/ICC detected inHeLa cells, MCF-7 cells, U2OS cells, HepG2 cells, A431 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy KI67 Ready-To-Use IHC Kit. KI67 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84192-4-RR targets Ki-67 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name antigen identified by monoclonal antibody Ki-67
Synonyms MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67
Calculated Molecular Weight 359 kDa
GenBank Accession NumberNM_002417
Gene Symbol KI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP46013
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

实验方案

Product Specific Protocols
WB protocol for Ki-67 antibody 84192-4-RRDownload protocol
IHC protocol for Ki-67 antibody 84192-4-RRDownload protocol
IF protocol for Ki-67 antibody 84192-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols