验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, mouse spleen tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human lymphoma tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13025-1-AP targets L-Plastin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey, zebrafish, hamster |
| Immunogen | L-Plastin fusion protein Ag3571 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lymphocyte cytosolic protein 1 (L-plastin) |
| Synonyms | L PLASTIN, LCP 1, LCP1, L-Plastin, Lymphocyte cytosolic protein 1, Plastin 2, PLS2 |
| Calculated Molecular Weight | 627 aa, 64 kDa |
| Observed Molecular Weight | 64-70 kDa |
| GenBank Accession Number | BC010271 |
| Gene Symbol | L-Plastin |
| Gene ID (NCBI) | 3936 |
| RRID | AB_2136871 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13796 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for L-Plastin antibody 13025-1-AP | Download protocol |
| IHC protocol for L-Plastin antibody 13025-1-AP | Download protocol |
| IP protocol for L-Plastin antibody 13025-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Signal A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity | ||
Mol Ther Nucleic Acids Exosomal Transfer of LCP1 Promotes Osteosarcoma Cell Tumorigenesis and Metastasis by Activating the JAK2/STAT3 Signaling Pathway. | ||
J Cell Sci Iterative Bleaching Extends Multiplexity (IBEX) facilitates simultaneous identification of all major retinal cell types | ||
Cancer Biomark LCP1 is a prognostic biomarker correlated with immune infiltrates in gastric cancer | ||
Drug Dev Res Therapeutic role of Artemether in the prevention of hepatic steatosis through miR-34a-5p/PPARα pathway | ||
