验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, K-562 cells, mouse spleen tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human lymphoma tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13025-1-AP targets L-Plastin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, monkey, zebrafish, hamster |
免疫原 | L-Plastin fusion protein Ag3571 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | lymphocyte cytosolic protein 1 (L-plastin) |
别名 | L PLASTIN, LCP 1, LCP1, L-Plastin, Lymphocyte cytosolic protein 1, Plastin 2, PLS2 |
计算分子量 | 627 aa, 64 kDa |
观测分子量 | 64-70 kDa |
GenBank蛋白编号 | BC010271 |
基因名称 | L-Plastin |
Gene ID (NCBI) | 3936 |
RRID | AB_2136871 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P13796 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for L-Plastin antibody 13025-1-AP | Download protocol |
IHC protocol for L-Plastin antibody 13025-1-AP | Download protocol |
IP protocol for L-Plastin antibody 13025-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Signal A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity | ||
Mol Ther Nucleic Acids Exosomal Transfer of LCP1 Promotes Osteosarcoma Cell Tumorigenesis and Metastasis by Activating the JAK2/STAT3 Signaling Pathway. | ||
J Cell Sci Iterative Bleaching Extends Multiplexity (IBEX) facilitates simultaneous identification of all major retinal cell types | ||
Cancer Biomark LCP1 is a prognostic biomarker correlated with immune infiltrates in gastric cancer | ||
Drug Dev Res Therapeutic role of Artemether in the prevention of hepatic steatosis through miR-34a-5p/PPARα pathway | ||