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验证数据展示

  • WB analysis using 21799-1-AP

    WB analysis using 21799-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21799-1-AP (LDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 21799-1-AP

    IHC staining of human skeletal muscle using 21799-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21799-1-AP (LDHA antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 21799-1-AP

    IHC staining of human skeletal muscle using 21799-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21799-1-AP (LDHA antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HEK-293 using 21799-1-AP

    IP experiment of HEK-293 using 21799-1-AP

    IP result of anti-LDHA (IP:21799-1-AP, 4ug; Detection:21799-1-AP 1:500) with HEK-293 cells lysate 2000ug.

  • WB analysis of MDA-MB-231 using 21799-1-AP

    WB analysis of MDA-MB-231 using 21799-1-AP

    WB result of LDHA antibody (21799-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells.

  • IF Staining of HepG2 using 21799-1-AP

    IF Staining of HepG2 using 21799-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA antibody (21799-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

LDHA Polyclonal antibody

LDHA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21799-1-AP

产品说明书

引用文献(61)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

LDH-M, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, human testis tissue, A375 cells, HepG2 cells, NIH/3T3 cells, MDA-MB-231 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:30000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21799-1-AP targets LDHA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, monkey
免疫原 LDHA fusion protein Ag16703 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lactate dehydrogenase A
别名 LDH-M, LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27
计算分子量 332 aa, 37 kDa
观测分子量 32-37 kDa
GenBank蛋白编号BC067223
基因名称 LDHA
Gene ID (NCBI) 3939
RRIDAB_10858925
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00338
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087).

实验方案

Product Specific Protocols
WB protocol for LDHA antibody 21799-1-APDownload protocol
IHC protocol for LDHA antibody 21799-1-APDownload protocol
IF protocol for LDHA antibody 21799-1-APDownload protocol
IP protocol for LDHA antibody 21799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

A tissue injury sensing and repair pathway distinct from host pathogen defense

Authors - Siqi Liu
View Article
mouseIF

Nat Metab

Time of exercise differentially impacts bone growth in mice

Authors - Shaoling Yu
View Article
humanWB

Sci Transl Med

Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.

Authors - Biying Zhu
View Article
humanWB,IF,IP

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
View Article
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
View Article

Cancer Res

HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.

Authors - Yinghui Li
View Article