验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HeLa cells, HEK-293T cells, Jurkat cells, PC-3 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19988-1-AP targets LDHB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lactate dehydrogenase B |
| 别名 | lactate dehydrogenase B, LDHB, EC:1.1.1.27, LDHA, LDH H |
| 计算分子量 | 37 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | NM_002300 |
| 基因名称 | LDHB |
| Gene ID (NCBI) | 3945 |
| RRID | AB_10638780 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07195 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LDHB, also named as NY-REN-46 and LDH-H, belongs to the LDH/MDH superfamily and LDH family. LDHB catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. It is glycolytic enzymes and it is positively regulated by mTOR.Defects in LDHB are a cause of hereditary LDHB deficiency. The antibody is specific to LDHB and has no cross reaction with LDHA and LDHC.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| IHC protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| IF protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomedicines HMTM-Mediated Enhancement of Brain Bioenergetics in a Mouse Tauopathy Model Is Blocked by Chronic Administration of Rivastigmine. | ||
J Nutr Sci Vitaminol (Tokyo) Combination of Exercise and Intake of Amino Acid Mixture Synergistically Induces Beige Adipocyte Formation in Mice | ||
Mol Biol Rep Development of a one-plasmid system to replace the endogenous protein with point mutation for post-translational modification studies. | ||
Am J Physiol Renal Physiol Expression of lactate dehydrogenase A and B isoforms in the mouse kidney. | ||
iScience Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma
| ||
iScience Altered neuronal lactate dehydrogenase A expression affects cognition in a sex- and age-dependent manner |