验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, mouse liver tissue, NIH/3T3 cells, Y79 cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 2 publications below |
产品信息
27102-1-AP targets LIMPII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | LIMPII fusion protein Ag25908 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | scavenger receptor class B, member 2 |
别名 | AMRF, CD36 antigen like 2, CD36L2, HLGP85, LGP85, LIMP II, limp2, LIMPII, Lysosome membrane protein 2, Lysosome membrane protein II, SCARB2, SR BII |
计算分子量 | 478 aa, 54 kDa |
观测分子量 | 75-85 kDa |
GenBank蛋白编号 | BC021892 |
基因名称 | SCARB2 |
Gene ID (NCBI) | 950 |
RRID | AB_2880756 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q14108 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for LIMPII antibody 27102-1-AP | Download protocol |
IHC protocol for LIMPII antibody 27102-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Virus Res Murine models of neonatal susceptibility to a clinical strain of enterovirus A71 | ||
Viruses A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice.
| ||
Infect Genet Evol Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body.
| ||
J Neurovirol Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells. | ||
PLoS Pathog The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness |