验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse liver tissue, NIH/3T3 cells, Y79 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27102-1-AP targets LIMPII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag25908 Product name: Recombinant human LIMPII protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 249-433 aa of BC021892 Sequence: NGTDGDSFHPLITKDEVLYVFPSDFCRSVYITFSDYESVQGLPAFRYKVPAEILANTSDNAGFCIPEGNCLGSGVLNVSICKNGAPIIMSFPHFYQADERFVSAIEGMHPNQEDHETFVDINPLTGIILKAAKRFQINIYVKKLDDFVETGDIRTMVFPVMYLNESVHIDKETASRLKSMINTTL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | scavenger receptor class B, member 2 |
| 别名 | AMRF, CD36 antigen like 2, CD36L2, HLGP85, LGP85, LIMP II, limp2, LIMPII, Lysosome membrane protein 2, Lysosome membrane protein II, SCARB2, SR BII |
| 计算分子量 | 478 aa, 54 kDa |
| 观测分子量 | 75-85 kDa |
| GenBank蛋白编号 | BC021892 |
| 基因名称 | SCARB2 |
| Gene ID (NCBI) | 950 |
| RRID | AB_2880756 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14108 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for LIMPII antibody 27102-1-AP | Download protocol |
| WB protocol for LIMPII antibody 27102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Activation of Lysosomal Retrograde Transport Triggers TPC1-IP3R1 Ca2+ Crosstalk at Lysosome-ER MCSs Leading to Lethal Depleting of ER Calcium | ||
PLoS Pathog The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness | ||
Virol Sin In vivo quantitative proteomic analysis of porcine alveolar macrophages in PRRSV-infected pigs
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