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验证数据展示

  • IF Staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    IF Staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using LIN28 antibody (60344-1-Ig, Clone: 4F5G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

    WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

LIN28A Monoclonal antibody, PBS Only

LIN28A Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60344-1-PBS

产品说明书

CloneNo. 4F5G6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

Lin-28A, LIN28, Lin 28A, LIN 28, CSDD1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

60344-1-PBS targets LIN28A in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2312

Product name: Recombinant human LIN28 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-209 aa of BC028566

Sequence: MGSVSNQQFAGGCAKAAEEAPEEAPEDAARAADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGPSAQGKPTYFREEEEEIHSPTLLPEAQN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lin-28 homolog (C. elegans)
别名 Lin-28A, LIN28, Lin 28A, LIN 28, CSDD1
计算分子量 209 aa, 23 kDa
观测分子量 28 kDa
GenBank蛋白编号BC028566
基因名称 LIN28
Gene ID (NCBI) 79727
RRIDAB_2881453
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9H9Z2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. LIN28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic mRNA-binding protein that binds to and enhances the translation of the IGF2 mRNA. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

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