LOXL2 Monoclonal antibody
LOXL2 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, rat and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1H7F1
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, human placenta tissue, A375 cells, human heart tissue |
| Positive IHC detected in | rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy LOXL2 Ready-To-Use IHC Kit. LOXL2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 2 publications below |
产品信息
67139-1-Ig targets LOXL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.
| Tested Applications | WB, IF, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human, rat |
| Cited Reactivity | human, mouse |
| Immunogen | LOXL2 fusion protein Ag28566 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lysyl oxidase-like 2 |
| Synonyms | LOR2, LOXL2, Lysyl oxidase homolog 2, lysyl oxidase like 2, Lysyl oxidase like protein 2, WS9 14 |
| Calculated Molecular Weight | 774 aa, 87 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC000594 |
| Gene Symbol | LOXL2 |
| Gene ID (NCBI) | 4017 |
| RRID | AB_2882438 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y4K0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. We detected a band about 100 kd which is a secretory mature LOXL2 protein modified by glycosylation (PMID: 24014025).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LOXL2 antibody 67139-1-Ig | Download protocol |
| IHC protocol for LOXL2 antibody 67139-1-Ig | Download protocol |
| IF protocol for LOXL2 antibody 67139-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest LOXL2-induced PEAR1 Ser891 phosphorylation suppresses CD44 degradation and promotes triple-negative breast cancer metastasis | ||
Aging Cell Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis. | ||
Int J Biol Macromol Depletion of LOXL2 improves respiratory capacity: From air-breathing fish to mammal under hypoxia. | ||
Oncogene METTL1/WDR4-mediated tRNA m7G modification and mRNA translation control promote oncogenesis and doxorubicin resistance | ||
Neuron Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier |
