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LY6D Polyclonal antibody

LY6D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17361-1-AP

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Synonyms

Lymphocyte antigen 6D, Ly-6D, Ly 6D, E48 antigen, E48



经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inhuman skin cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17361-1-AP targets LY6D in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen LY6D fusion protein Ag10714 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphocyte antigen 6 complex, locus D
Synonyms Lymphocyte antigen 6D, Ly-6D, Ly 6D, E48 antigen, E48
Calculated Molecular Weight 128 aa, 13 kDa
Observed Molecular Weight 13-14 kDa
GenBank Accession NumberBC031330
Gene Symbol LY6D
Gene ID (NCBI) 8581
RRIDAB_2878397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14210
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.

实验方案

Product Specific Protocols
WB protocol for LY6D antibody 17361-1-APDownload protocol
IHC protocol for LY6D antibody 17361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIF

Nat Commun

AP-1 and TGFß cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma.

Authors - Catherine D Yao
mouseIF,IHC

Cell Rep

Autocrine activation of MAPK signaling mediates intrinsic tolerance to androgen deprivation in LY6D prostate cancer cells

Authors - Ivana Steiner
mouseWB

Diabetes

PMID: 28325853

Authors - Yu-Yao Zhang
mouseIF

Elife

RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development.

Authors - Renaud Mevel
human,mouseIHC,IF

Cancers (Basel)

Lung Adenocarcinoma Mouse Models Based on Orthotopic Transplantation of Syngeneic Tumor-Initiating Cells Expressing EpCAM, SCA-1, and Ly6d.

Authors - Takashi Semba
  • KD Validated
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao