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LY6D Polyclonal antibody

LY6D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17361-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

E48, E48 antigen, Ly 6D, Ly-6D, Lymphocyte antigen 6D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inhuman skin cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17361-1-AP targets LY6D in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag10714

Product name: Recombinant human LY6D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-102 aa of BC031330

Sequence: MRTALLLLATLAVATGPALTLRCHVCTSSSNCKHSVVCPASSRFCKTTNTVEPLRGNLVKKDCAESCTPSYTLQGQVSSGTSSTQCCQEDLCNEKLHNAAPT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte antigen 6 complex, locus D
别名 E48, E48 antigen, Ly 6D, Ly-6D, Lymphocyte antigen 6D
计算分子量 128 aa, 13 kDa
观测分子量 13-14 kDa
GenBank蛋白编号BC031330
基因名称 LY6D
Gene ID (NCBI) 8581
RRIDAB_2878397
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14210
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.

实验方案

Product Specific Protocols
WB protocol for LY6D antibody 17361-1-APDownload protocol
IHC protocol for LY6D antibody 17361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIF

Nat Commun

AP-1 and TGFß cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma.

Authors - Catherine D Yao
mouseIHC,IF

Cell Rep

Autocrine activation of MAPK signaling mediates intrinsic tolerance to androgen deprivation in LY6D prostate cancer cells

Authors - Ivana Steiner
mouseWB

Diabetes

PMID: 28325853

Authors - Yu-Yao Zhang
mouseIF

Elife

RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development.

Authors - Renaud Mevel
mouse,humanIHC,IF

Cancers (Basel)

Lung Adenocarcinoma Mouse Models Based on Orthotopic Transplantation of Syngeneic Tumor-Initiating Cells Expressing EpCAM, SCA-1, and Ly6d.

Authors - Takashi Semba
  • KD Validated
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
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