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验证数据展示

  • WB analysis using 80906-1-RR (same clone as 80906-1-PBS)

    WB analysis using 80906-1-RR (same clone as 80906-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80906-1-RR (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80906-1-RR (same clone as 80906-1-PBS)

    WB analysis of HeLa using 80906-1-RR (same clone as 80906-1-PBS)

    WB result of Lamin B1 antibody (80906-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells. This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 80906-1-RR (same clone as 80906-1-PBS)

    IHC staining of human breast cancer using 80906-1-RR (same clone as 80906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin B1 antibody included
  • IF Staining of HepG2 using 80906-1-RR (same clone as 80906-1-PBS)

    IF Staining of HepG2 using 80906-1-RR (same clone as 80906-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B1 antibody (80906-1-RR, Clone: 5H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80906-1-RR (same clone as 80906-1-PBS)

    IF Staining of HepG2 using 80906-1-RR (same clone as 80906-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B1 antibody (80906-1-RR, Clone: 5H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80906-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80906-1-RR

    Affinity and Kinetic Characterization of 80906-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80906-1-RR against Human Lamin B1 were performed. The affinity constant is 13.8 nM.

  • Featured Product
  • KD/KO验证

Lamin B1 Recombinant antibody, PBS Only

Lamin B1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80906-1-PBS

产品说明书

CloneNo. 5H10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Lamin B1 Ready-To-Use IHC Kit. Lamin B1 primary antibody included.

产品信息

80906-1-PBS targets Lamin B1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Lamin B1 fusion protein Ag3631 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lamin B1
别名 LMNB1, LMNB, LMN2, Lamin-B1, laminB1
计算分子量 66 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC012295
基因名称 Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
RRIDAB_2918918
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20700
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.