验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, A431 cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue, mouse small intestine tissue, mouse pancreas tissue, rat kidney tissue, mouse kidney tissue, rat pancreas tissue, human placenta tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy® MAGED2 Ready-To-Use IHC Kit. MAGED2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15252-1-AP targets MAGED2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6828 Product name: Recombinant human MAGED2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-479 aa of BC000304 Sequence: DEPEPESAAAQSQENQDTRPKVKAKKARKVKHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARRALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPIKRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGILGTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEFVKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | melanoma antigen family D, 2 |
| 别名 | 11B6, BCG-1, Breast cancer-associated gene 1 protein, HCA10, Hepatocellular carcinoma-associated protein JCL-1 |
| 计算分子量 | 65 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC000304 |
| 基因名称 | MAGED2 |
| Gene ID (NCBI) | 10916 |
| RRID | AB_2138557 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNF1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MAGED2 antibody 15252-1-AP | Download protocol |
| IHC protocol for MAGED2 antibody 15252-1-AP | Download protocol |
| IP protocol for MAGED2 antibody 15252-1-AP | Download protocol |
| WB protocol for MAGED2 antibody 15252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nephrol Dial Transplant MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells. | ||
Biochem Pharmacol Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response.
| ||
mBio SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense
| ||
J Neurosci MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation. | ||
Biochim Biophys Acta Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.
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