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- KD/KO Validated
MAGED2 Polyclonal antibody
MAGED2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, A431 cells, MCF-7 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15252-1-AP targets MAGED2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IP, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MAGED2 fusion protein Ag6828 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | melanoma antigen family D, 2 |
| Synonyms | JCL 1, Hepatocellular carcinoma-associated protein JCL-1, HCA10, Breast cancer-associated gene 1 protein, BCG-1 |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC000304 |
| Gene Symbol | MAGED2 |
| Gene ID (NCBI) | 10916 |
| RRID | AB_2138557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNF1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAGED2 antibody 15252-1-AP | Download protocol |
| IHC protocol for MAGED2 antibody 15252-1-AP | Download protocol |
| IF protocol for MAGED2 antibody 15252-1-AP | Download protocol |
| IP protocol for MAGED2 antibody 15252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nephrol Dial Transplant MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells. | ||
J Neurosci MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation. | ||
Biochem Pharmacol Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response.
| ||
Biochim Biophys Acta Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.
| ||
mBio SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense
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