验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18726-1-AP targets LC3C-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 1 light chain 3 gamma |
| 别名 | LC3C, MAP1A/MAP1B LC3 C, MAP1A/MAP1B light chain 3 C, MAP1LC3C |
| 计算分子量 | 17 kDa |
| GenBank蛋白编号 | NM_001004343 |
| 基因名称 | LC3C |
| Gene ID (NCBI) | 440738 |
| RRID | AB_2878557 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXW4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LC3C, also named as MAP1LC3C, belongs to the MAP1 LC3 family. It is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3C is produced by the proteolytic cleavage after the conserved C-terminal Gly residue. The antibody is specific to the prepeptide of LC3C.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for LC3C-Specific antibody 18726-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. | ||
J Immunol Cutting Edge: Phagosome-associated Autophagosomes Containing Antigens and Proteasomes Drive TAP-Independent Cross-Presentation | ||
Front Pharmacol SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway | ||
PLoS One Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines. | ||
Adv Exp Med Biol Microtubule-Associated Protein 1 Light Chain 3 (LC3) Isoforms in RPE and Retina. | ||
Dev Cell V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation |
