验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18726-1-AP targets LC3C-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | microtubule-associated protein 1 light chain 3 gamma |
| Synonyms | LC3C, MAP1A/MAP1B LC3 C, MAP1A/MAP1B light chain 3 C, MAP1LC3C |
| Calculated Molecular Weight | 17 kDa |
| GenBank Accession Number | NM_001004343 |
| Gene Symbol | LC3C |
| Gene ID (NCBI) | 440738 |
| RRID | AB_2878557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXW4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LC3C, also named as MAP1LC3C, belongs to the MAP1 LC3 family. It is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3C is produced by the proteolytic cleavage after the conserved C-terminal Gly residue. The antibody is specific to the prepeptide of LC3C.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for LC3C-Specific antibody 18726-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. | ||
J Immunol Cutting Edge: Phagosome-associated Autophagosomes Containing Antigens and Proteasomes Drive TAP-Independent Cross-Presentation | ||
Front Pharmacol SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway | ||
PLoS One Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines. | ||
Adv Exp Med Biol Microtubule-Associated Protein 1 Light Chain 3 (LC3) Isoforms in RPE and Retina. | ||
Dev Cell V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation |
