验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human prostate cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15695-1-AP targets MAP1S in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | MAP1S fusion protein Ag8315 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 1S |
| 别名 | BPY2 interacting protein 1, BPY2IP1, C19orf5, MAP 1S, MAP1S, MAP8, VCY2 interacting protein 1, VCY2IP 1, VCY2IP1 |
| 计算分子量 | 806 aa, 85 kDa |
| 观测分子量 | 130-150 kDa |
| GenBank蛋白编号 | BC008806 |
| 基因名称 | MAP1S |
| Gene ID (NCBI) | 55201 |
| RRID | AB_2137868 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q66K74 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP1S (also known as C19ORF5 or VCY2IP1) is a novel member of the microtubule-associated protein 1 family and a homologue of the exclusively neuronal distributed microtubule-associated protein 1A and 1B (MAP1A/B). In contrast to MAP1A and MAP1B, MAP1S is expressed in a wide range of tissues in addition to neurons. MAP1S is synthesized as a precursor protein that is partially cleaved into heavy and light chains in a tissue-specific manner. In addition, a short chain isoform may be induced under prolonged mitotic arrest or inhibition of the 26S proteasome. Recently it has been reported that the short chain isoform associates with mitochondria in addition to microtubules and causes irreversible aggregation of dysfunctional mitochondria resulting in cell death. Western blot analysis in human brain using this antibody detected two main bands between 100-130 kDa corresponding to heavy and light chains of MAP1S.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAP1S antibody 15695-1-AP | Download protocol |
| IHC protocol for MAP1S antibody 15695-1-AP | Download protocol |
| IF protocol for MAP1S antibody 15695-1-AP | Download protocol |
| IP protocol for MAP1S antibody 15695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly. | ||
EMBO Rep MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules. | ||
Cell Cycle Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability. | ||
Front Synaptic Neurosci Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction. | ||
Biochim Biophys Acta Mol Cell Res Cryptic splicing events result in unexpected protein products from calpain-10 (CAPN10) cDNA | ||
Front Pharmacol Lipid droplets: a candidate new research field for epithelial ovarian cancer |
