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MAP2K4 Polyclonal antibody

MAP2K4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17340-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, RAW264.7, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17340-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 MAP2K4 fusion protein Ag11376 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 4
别名 JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1
计算分子量 399 aa, 44 kDa
观测分子量44-50 kDa
GenBank蛋白编号BC036032
基因名称 MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2878389
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP45985
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MAP2K4 antibody 17340-1-APDownload protocol
IHC protocol for MAP2K4 antibody 17340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
mouse,ratWB

J Hazard Mater

Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.

Authors - Junrong Wu
mouseWB

J Cell Mol Med

NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.

Authors - Ze-Qing Pu
ratWB

Cells

Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.

Authors - Elaheh Soleimanpour
humanWB

Int J Biochem Cell Biol

Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.

Authors - Xuechao Wan
  • KD Validated
humanWB

Dig Dis Sci

LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4.

Authors - Jianhui Liu
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