验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells, RAW264.7, RAW 264.7 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17340-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | MAP2K4 fusion protein Ag11376 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase kinase 4 |
| Synonyms | JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1 |
| Calculated Molecular Weight | 399 aa, 44 kDa |
| Observed Molecular Weight | 44-50 kDa |
| GenBank Accession Number | BC036032 |
| Gene Symbol | MAP2K4 |
| Gene ID (NCBI) | 6416 |
| RRID | AB_2878389 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAP2K4 antibody 17340-1-AP | Download protocol |
| IHC protocol for MAP2K4 antibody 17340-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. | ||
J Cell Mol Med NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection. | ||
Int J Biochem Cell Biol Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.
| ||
Dig Dis Sci LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4. |
