验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HeLa cells, RAW264.7, RAW 264.7 cells |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17340-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | MAP2K4 fusion protein Ag11376 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | mitogen-activated protein kinase kinase 4 |
别名 | JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1 |
计算分子量 | 399 aa, 44 kDa |
观测分子量 | 44-50 kDa |
GenBank蛋白编号 | BC036032 |
基因名称 | MAP2K4 |
Gene ID (NCBI) | 6416 |
RRID | AB_2878389 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P45985 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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WB protocol for MAP2K4 antibody 17340-1-AP | Download protocol |
IHC protocol for MAP2K4 antibody 17340-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. | ||
J Cell Mol Med NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection. | ||
Int J Biochem Cell Biol Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.
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Dig Dis Sci LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4. |