JNK Polyclonal antibody
JNK Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
17337-1-AP targets JNK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | JNK fusion protein Ag11372 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase 9 |
| Synonyms | MAPK9, JNK2A, JNK2, JNK 55, EC:2.7.11.24 |
| Calculated Molecular Weight | 424 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC032539 |
| Gene Symbol | JNK |
| Gene ID (NCBI) | 5601 |
| RRID | AB_2878388 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45984 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for JNK antibody 17337-1-AP | Download protocol |
| IF protocol for JNK antibody 17337-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol RG108 attenuates acute kidney injury by inhibiting P38 MAPK/FOS and JNK/JUN pathways | ||
Adv Sci (Weinh) Ultra-Long-Term Anti-Inflammatory Polyphenol Capsule to Remodel the Microenvironment for Accelerating Osteoarthritis Healing by Single Dosage | ||
BMC Cancer Aldo-keto reductase family 1 member C3 mediates radioresistance of esophageal cancer cells through suppressing MAPK and AKT signaling |