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MAPKAPK3 Polyclonal antibody

MAPKAPK3 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15424-1-AP

Print datasheet

Synonyms

3PK, Chromosome 3p kinase, MAPKAP kinase 3, MAPKAP3, MAPKAPK 3, MAPKAPK3

验证数据展示

  • WB analysis of mouse skeletal muscle using 15424-1-AP

    WB analysis of mouse skeletal muscle using 15424-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15424-1-AP (MAPKAPK3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 15424-1-AP

    IHC staining of human placenta using 15424-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 15424-1-AP

    IHC staining of human placenta using 15424-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15424-1-AP

    IF Staining of HeLa using 15424-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15424-1-AP targets MAPKAPK3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen MAPKAPK3 fusion protein Ag7875 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase-activated protein kinase 3
Synonyms 3PK, Chromosome 3p kinase, MAPKAP kinase 3, MAPKAP3, MAPKAPK 3, MAPKAPK3
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001662
Gene Symbol MAPKAPK3
Gene ID (NCBI) 7867
RRIDAB_2878136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16644
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAPKAPK3 antibody 15424-1-APDownload protocol
IHC protocol for MAPKAPK3 antibody 15424-1-APDownload protocol
IF protocol for MAPKAPK3 antibody 15424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Bioengineered

Inhibition of translocator protein 18 kDa suppressed the progression of glioma via the ELAV-like RNA-binding protein 1/MAPK-activated protein kinase 3 axis.

Authors - Jingya Wang
View Article
ratWB,IF

Front Neurosci

Identification of key genes and pathways revealing the central regulatory mechanism of brain-derived glucagon-like peptide-1 on obesity using bioinformatics analysis

Authors - Yuwei Shao
View Article