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验证数据展示

  • WB analysis of mouse skeletal muscle using 15424-1-AP

    WB analysis of mouse skeletal muscle using 15424-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15424-1-AP (MAPKAPK3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 15424-1-AP

    IHC staining of human placenta using 15424-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 15424-1-AP

    IHC staining of human placenta using 15424-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15424-1-AP

    IF Staining of HeLa using 15424-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAPKAPK3 Polyclonal antibody

MAPKAPK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15424-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Synonyms

MAPKAP kinase 3, MAPK-activated protein kinase 3, MAP kinase-activated protein kinase 3, EC:2.7.11.1, Chromosome 3p kinase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15424-1-AP targets MAPKAPK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen MAPKAPK3 fusion protein Ag7875 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase-activated protein kinase 3
Synonyms MAPKAP kinase 3, MAPK-activated protein kinase 3, MAP kinase-activated protein kinase 3, EC:2.7.11.1, Chromosome 3p kinase
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001662
Gene Symbol MAPKAPK3
Gene ID (NCBI) 7867
RRIDAB_2878136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16644
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAPKAPK3 antibody 15424-1-APDownload protocol
IHC protocol for MAPKAPK3 antibody 15424-1-APDownload protocol
IF protocol for MAPKAPK3 antibody 15424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Bioengineered

Inhibition of translocator protein 18 kDa suppressed the progression of glioma via the ELAV-like RNA-binding protein 1/MAPK-activated protein kinase 3 axis.

Authors - Jingya Wang
View Article
ratWB,IF

Front Neurosci

Identification of key genes and pathways revealing the central regulatory mechanism of brain-derived glucagon-like peptide-1 on obesity using bioinformatics analysis

Authors - Yuwei Shao
View Article