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MCM2 Polyclonal antibody

MCM2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat no : 10513-1-AP

Print datasheet

Synonyms

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

验证数据展示

WB analysis using 10513-1-AP
Various cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10513-1-AP
WB result of MCM2 antibody (10513-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells.
IF Staining of HeLa using 10513-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (10513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IP experiment of HEK-293 using 10513-1-AP
IP result of anti-MCM2 (IP:10513-1-AP, 4ug; Detection:10513-1-AP 1:800) with HEK-293 cells lysate 2800ug.
IHC staining of human cervical cancer using 10513-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MCM2 antibody included
IF Staining of human gliomas using 10513-1-AP
Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 10513-1-AP (MCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human cervical cancer using 10513-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MCM2 antibody included
FC experiment of NIH/3T3 using 10513-1-AP
1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human MCM2 (10513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 10513-1-AP

Size

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经过测试的应用

Positive WB detected in	HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human cervical cancer tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human gliomas tissue
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	NIH/3T3 cells

Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 33 publications below
IHC	See 11 publications below
IF	See 4 publications below
IP	See 1 publications below
RIP	See 1 publications below

产品信息

10513-1-AP targets MCM2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, RIP
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Immunogen	MCM2 fusion protein Ag0798 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	minichromosome maintenance complex component 2
Synonyms	KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28
Calculated Molecular Weight	102 kDa
Observed Molecular Weight	116-125 kDa
GenBank Accession Number	BC007670
Gene Symbol	MCM2
Gene ID (NCBI)	4171
RRID	AB_2142131
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49736
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

实验方案

Product Specific Protocols
WB protocol for MCM2 antibody 10513-1-AP	Download protocol
IHC protocol for MCM2 antibody 10513-1-AP	Download protocol
IF protocol for MCM2 antibody 10513-1-AP	Download protocol
IP protocol for MCM2 antibody 10513-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB,IP	Nature CDC7-independent G1/S transition revealed by targeted protein degradation. Authors - Jan M Suski View Article
human	WB	Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation Authors - Xiaohua Xu View Article
human	IF	Nat Commun Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes Authors - Hana Polasek-Sedlackova View Article
	WB	Cancer Res LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity Authors - Shiyi Yu View Article
human	WB,RIP	Int J Biol Sci ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway Authors - Shasha Li View Article
human	WB	Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. Authors - Hongyuan Song View Article

MCM2 Polyclonal antibody

MCM2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

验证数据展示

WB analysis using 10513-1-AP

WB analysis of HEK-293 using 10513-1-AP

IF Staining of HeLa using 10513-1-AP

IP experiment of HEK-293 using 10513-1-AP

IHC staining of human cervical cancer using 10513-1-AP

IF Staining of human gliomas using 10513-1-AP

IHC staining of human cervical cancer using 10513-1-AP

FC experiment of NIH/3T3 using 10513-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

CDC7-independent G1/S transition revealed by targeted protein degradation.

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes

LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity

ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

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