验证数据展示
经过测试的应用
| Positive IHC detected in | human skin cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
15734-1-AP targets MIA in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag8499 Product name: Recombinant human MIA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-131 aa of BC005910 Sequence: GGPMPKLADRKLCADQECSHPISMAVALQDYMAPDCRFLTIHRGQVVYVFSKLKGRGRLFWGGSVQGDYYGDLAARLGYFPSSIVREDQTLKPGKVDVKTDKWDFYCQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | melanoma inhibitory activity |
| 别名 | CD RAP, melanoma inhibitory activity, MIA |
| 计算分子量 | 131 aa, 15 kDa |
| GenBank蛋白编号 | BC005910 |
| 基因名称 | MIA |
| Gene ID (NCBI) | 8190 |
| RRID | AB_2878176 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16674 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Melanoma-derived growth regulatory protein, also known as MIA, CD RAP, is a protein that in humans is encoded by the MIA gene. It is a marker for malignant melanoma (PMID: 9242442).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MIA antibody 15734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
