Neprilysin/CD10 Polyclonal antibody

Neprilysin/CD10 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18008-1-AP

Print datasheet

Synonyms

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11



经过测试的应用

Positive WB detected inmouse kidney tissue, human kidney tissue, rat kidney tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18008-1-AP targets Neprilysin/CD10 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Neprilysin/CD10 fusion protein Ag12506 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane metallo-endopeptidase
Synonyms MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11
Calculated Molecular Weight80aa,9 kDa; 750aa,85 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC106070
Gene Symbol CD10
Gene ID (NCBI) 4311
RRIDAB_2146541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08473
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

实验方案

Product Specific Protocols
WB protocol for Neprilysin/CD10 antibody 18008-1-APDownload protocol
IHC protocol for Neprilysin/CD10 antibody 18008-1-APDownload protocol
IF protocol for Neprilysin/CD10 antibody 18008-1-APDownload protocol
IP protocol for Neprilysin/CD10 antibody 18008-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Adv Sci (Weinh)

Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression

Authors - Rui Zhou
humanWB

Brain

MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.

Authors - Bing-Lin Zhu
humanWB

Int J Biol Macromol

A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.

Authors - Hui Zeng
mouseWB

Mol Neurobiol

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Authors - Yasuteru Inoue
humanIHC

BMC Genomics

Genetic control of renal tumorigenesis by the mouse Rtm1 locus.

Authors - Jensen José Ricardo JR
mouseWB

J Alzheimers Dis

Dendrobium nobile Lindl. Alkaloids Ameliorate Cognitive Dysfunction in Senescence Accelerated SAMP8 Mice by Decreasing Amyloid-β Aggregation and Enhancing Autophagy Activity.

Authors - Ling-Li Lv