MMP13 Polyclonal antibody

MMP13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 18165-1-AP

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Synonyms

CLG3, Matrix metalloproteinase 13, MMP 13, MMP13



经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, MDA-MB-453s cells, mouse liver tissue, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

18165-1-AP targets MMP13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, pig, rabbit, zebrafish
Immunogen MMP13 fusion protein Ag12653 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 13
Synonyms CLG3, Matrix metalloproteinase 13, MMP 13, MMP13
Calculated molecular weight 471 aa, 54 kDa
Observed molecular weight 65-70 kDa, 50-55 kDa, 40 kDa
GenBank accession numberBC074808
Gene symbol MMP13
UniprotP45452
Gene ID (NCBI) 4322
RRIDAB_2144858
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).

实验方案

Product Specific Protocols
WB protocol for MMP13 antibody 18165-1-APDownload protocol
IHC protocol for MMP13 antibody 18165-1-APDownload protocol
IF protocol for MMP13 antibody 18165-1-APDownload protocol
IP protocol for MMP13 antibody 18165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
mouse,ratWB,IF

Exp Mol Med

SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis

Authors - Jianxin Mao
mouseIF

Nat Commun

Mesenchymal stromal cell-derived septoclasts resorb cartilage during developmental ossification and fracture healing.

Authors - Kishor K Sivaraj
mouseWB,IHC

Nat Commun

Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.

Authors - Yun Zhang
humanIF

Bone Res

Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration.

Authors - Dong Wang
humanIHC

Bone Res

FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis.

Authors - Dong Guo