MMP13 Polyclonal antibody
MMP13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
274
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-453s cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
18165-1-AP targets MMP13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF, IHC, IP, WB, ELISA |
| Cited Applications | IF, IHC, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine, pig, rabbit, zebrafish |
| Immunogen | MMP13 fusion protein Ag12653 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | matrix metallopeptidase 13 |
| Synonyms | CLG3, Matrix metalloproteinase 13, MMP 13, MMP13 |
| Calculated molecular weight | 471 aa, 54 kDa |
| Observed molecular weight | 65-70 kDa, 50-55 kDa, 40 kDa |
| GenBank accession number | BC074808 |
| Gene symbol | MMP13 |
| Uniprot | P45452 |
| Gene ID (NCBI) | 4322 |
| RRID | AB_2144858 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MMP13 antibody 18165-1-AP | Download protocol |
| IHC protocol for MMP13 antibody 18165-1-AP | Download protocol |
| IF protocol for MMP13 antibody 18165-1-AP | Download protocol |
| IP protocol for MMP13 antibody 18165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Mol Med SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis | ||
Nat Commun Mesenchymal stromal cell-derived septoclasts resorb cartilage during developmental ossification and fracture healing. | ||
Nat Commun Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. | ||
Bone Res Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration. | ||
Bone Res FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis. |
