|Positive WB detected in||HeLa cells, MCF-7 cells, MDA-MB-453s cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18165-1-AP targets MMP13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||IF, IHC, IP, WB, ELISA|
|Cited Applications||IF, IHC, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig, rabbit, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MMP13 fusion protein Ag12653|
|Full Name||matrix metallopeptidase 13|
|Synonyms||CLG3, Matrix metalloproteinase 13, MMP 13, MMP13|
|Calculated molecular weight||471 aa, 54 kDa|
|Observed molecular weight||65-70 kDa, 50-55 kDa, 40 kDa|
|GenBank accession number||BC074808|
|Uniprot||P45452||Gene ID (NCBI)||4322|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).
|Product Specific Protocols|
|WB protocol for MMP13 antibody 18165-1-AP||Download protocol|
|IHC protocol for MMP13 antibody 18165-1-AP||Download protocol|
|IF protocol for MMP13 antibody 18165-1-AP||Download protocol|
|IP protocol for MMP13 antibody 18165-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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