|Positive WB detected in||MCF-7 cells, A375 cells, MDA-MB-453s cells, HepG2 cells, HT-1080 cells, A431 cells, SGC-7901 cells, U-251 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human gliomas tissue, human heart tissue, human breast cancer tissue, human placenta tissue, human oesophagus tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10373-2-AP targets MMP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Tested Applications||IF, IHC, IP, WB, ELISA|
|Cited Applications||IF, IHC, IP, WB|
|Cited Reactivity||human, mouse, rat, bovine, fish, pig, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MMP2 fusion protein Ag0549|
|Full Name||matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|
|Synonyms||72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MMP-2, MONA, TBE 1|
|Calculated molecular weight||72 kDa|
|Observed molecular weight||62-72 kDa|
|GenBank accession number||BC002576|
|Uniprot||P08253||Gene ID (NCBI)||4313|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).
|Product Specific Protocols|
|WB protocol for MMP2 antibody 10373-2-AP||Download protocol|
|IHC protocol for MMP2 antibody 10373-2-AP||Download protocol|
|IF protocol for MMP2 antibody 10373-2-AP||Download protocol|
|IP protocol for MMP2 antibody 10373-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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