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MMP2 Polyclonal antibody
MMP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (5)
Applications
IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
703
验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, U-251 cells, HepG2 cells, MCF-7 cells, U-87 MG cells, J774A.1 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human gliomas tissue, human placenta tissue, human prostate cancer tissue, human heart tissue, human oesophagus tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
In sample preparation, the extracellular matrix needs to be included
产品信息
10373-2-AP targets MMP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF, IHC, IP, WB, ELISA |
| Cited Applications | IF, IHC, IP, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, bovine, fish, pig, rabbit |
| Immunogen | MMP2 fusion protein Ag0549 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) |
| Synonyms | 72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MMP-2, MONA, TBE 1 |
| Calculated molecular weight | 72 kDa |
| Observed molecular weight | 62-72 kDa |
| GenBank accession number | BC002576 |
| Gene symbol | MMP2 |
| Uniprot | P08253 |
| Gene ID (NCBI) | 4313 |
| RRID | AB_2250823 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MMP2 antibody 10373-2-AP | Download protocol |
| IHC protocol for MMP2 antibody 10373-2-AP | Download protocol |
| IF protocol for MMP2 antibody 10373-2-AP | Download protocol |
| IP protocol for MMP2 antibody 10373-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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J Thorac Oncol Silencing NKD2 by Promoter Region Hypermethylation Promotes Esophageal Cancer Progression by Activating Wnt Signaling. | ||
Nat Commun Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling. | ||
