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MMP2 Polyclonal antibody

MMP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (5)





Cat No : 10373-2-AP

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72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MMP-2, MONA, TBE 1


Positive WB detected inHT-1080 cells, U-251 cells, HepG2 cells, MCF-7 cells, U-87 MG cells, J774A.1 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman gliomas tissue, human placenta tissue, human prostate cancer tissue, human heart tissue, human oesophagus tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MMP2 Ready-To-Use IHC Kit. MMP2 primary antibody included.


Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

In sample preparation, the extracellular matrix needs to be included


10373-2-AP targets MMP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, bovine, fish, pig, rabbit
Immunogen MMP2 fusion protein Ag0549 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Synonyms 72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MMP-2, MONA, TBE 1
Calculated molecular weight 72 kDa
Observed molecular weight62-72 kDa
GenBank accession numberBC002576
Gene symbol MMP2
Gene ID (NCBI) 4313
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).


Product Specific Protocols
WB protocol for MMP2 antibody 10373-2-APDownload protocol
IHC protocol for MMP2 antibody 10373-2-APDownload protocol
IF protocol for MMP2 antibody 10373-2-APDownload protocol
IP protocol for MMP2 antibody 10373-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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