验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, Raji cells |
| Positive IF/ICC detected in | Raji cells |
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 4 publications below |
产品信息
24828-1-AP targets CD20 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CD20 fusion protein Ag17309 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | membrane-spanning 4-domains, subfamily A, member 1 |
| 别名 | Leukocyte surface antigen Leu-16, Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1 |
| 计算分子量 | 297 aa, 33 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC002807 |
| 基因名称 | CD20 |
| Gene ID (NCBI) | 931 |
| RRID | AB_2879747 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11836 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD20 antibody 24828-1-AP | Download protocol |
| IHC protocol for CD20 antibody 24828-1-AP | Download protocol |
| IF protocol for CD20 antibody 24828-1-AP | Download protocol |
| IP protocol for CD20 antibody 24828-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunother Cancer Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors. | ||
Clin Exp Immunol CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome. | ||
Acta Physiol (Oxf) Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells. | ||
Int J Mol Sci CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing |
