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  • Featured Product
  • KD/KO Validated

MSH6 Monoclonal antibody, PBS Only

MSH6 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E10B2

Cat No : 66172-1-PBS

Print datasheet

Synonyms

G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160

验证数据展示

  • IHC staining of human colon cancer using 66172-1-Ig (same clone as 66172-1-PBS)

    IHC staining of human colon cancer using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH6 antibody included
  • IF Staining of U2OS using 66172-1-Ig (same clone as 66172-1-PBS)

    IF Staining of U2OS using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH6 antibody (66172-1-Ig, Clone: 2E10B2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    WB analysis of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    WB result of MSH6 antibody (66172-1-Ig, 1:1000) with si-Control and si-MSH6 transfected HEK293 cells. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    WB analysis of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    IP experiment of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    IP result of anti-MSH6 (IP:66172-1-Ig, 4ug; Detection:66172-1-Ig 1:600) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 66172-1-Ig (same clone as 66172-1-PBS)

    WB analysis of U2OS using 66172-1-Ig (same clone as 66172-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • WB analysis using 66172-1-Ig (same clone as 66172-1-PBS)

    WB analysis using 66172-1-Ig (same clone as 66172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MSH6 Ready-To-Use IHC Kit. MSH6 primary antibody included.

产品信息

66172-1-PBS targets MSH6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IF, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MSH6 fusion protein Ag12645 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mutS homolog 6 (E. coli)
Synonyms G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160
Calculated Molecular Weight 153 kDa
Observed Molecular Weight 160 kDa
GenBank Accession NumberBC004246
Gene Symbol MSH6
Gene ID (NCBI) 2956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52701
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.