MSI1 Polyclonal antibody

MSI1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27185-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

MSI1, Musashi 1, musashi homolog 1 (Drosophila)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, Caco-2 cells, HEK-293T cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MSI1 Ready-To-Use IHC Kit. MSI1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27185-1-AP targets MSI1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 MSI1 fusion protein Ag26042 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 musashi homolog 1 (Drosophila)
别名 MSI1, Musashi 1, musashi homolog 1 (Drosophila)
计算分子量 362 aa, 39 kDa
观测分子量 35 kDa
GenBank蛋白编号BC146463
基因名称 MSI1
Gene ID (NCBI) 4440
RRIDAB_2880790
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43347
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.

实验方案

Product Specific Protocols
WB protocol for MSI1 antibody 27185-1-APDownload protocol
IHC protocol for MSI1 antibody 27185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Oncol

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Authors - Wei Jia
humanIF

Front Mol Neurosci

Importin-Mediated Pathological Tau Nuclear Translocation Causes Disruption of the Nuclear Lamina, TDP-43 Mislocalization and Cell Death.

Authors - Robert F Candia
humanWB

J Cancer

PMID: 31031868

Authors - Huaizeng Chen
humanWB

Oncogene

Alternative splicing of ALDOA confers tamoxifen resistance in breast cancer

Authors - Shiyi Yu
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