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MUC4 Polyclonal antibody

MUC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28703-1-AP

Print datasheet

Synonyms

Mucin 4, MUC-4, MUC 4, ASGP 2, ASGP 1 Mucin 4 beta chain

验证数据展示

  • WB analysis using 28703-1-AP

    WB analysis using 28703-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28703-1-AP (MUC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 28703-1-AP

    IHC staining of human prostate cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC4 antibody included
  • IHC staining of human prostate cancer using 28703-1-AP

    IHC staining of human prostate cancer using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC4 antibody included
  • IHC staining of human colon using 28703-1-AP

    IHC staining of human colon using 28703-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC4 antibody included

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue, human colon tissue, human colon cancer tissue, human lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MUC4 Ready-To-Use IHC Kit. MUC4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28703-1-AP targets MUC4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MUC4 fusion protein Ag29799 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucin 4, cell surface associated
Synonyms Mucin 4, MUC-4, MUC 4, ASGP 2, ASGP 1 Mucin 4 beta chain
Calculated Molecular Weight 231 kDa
Observed Molecular Weight231 kDa, 113 kDa
GenBank Accession NumberNM_018406
Gene Symbol MUC4
Gene ID (NCBI) 4585
RRIDAB_2918192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99102
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MUC4, also named as HSA276359, ASGP, MUC-4, testis mucin and mucin-4 is a high molecular weight transmembrane mucin that is overexpressed in many carcinomas and is a risk factor associated with a poor prognosis. MUC4 have a key role in the progression of pancreatic cancer. MUC4 helps in the docking of tumor cells on the endothelial surface.

实验方案

Product Specific Protocols
WB protocol for MUC4 antibody 28703-1-APDownload protocol
IHC protocol for MUC4 antibody 28703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols