MYC-tag Monoclonal antibody
MYC-tag Monoclonal Antibody for WB, IP, IF, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Recombinant protein and More (3)
Applications
WB, IP, IF, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2D11A8
验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66004-1-Ig targets MYC-tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.
| Tested Applications | WB, IP, IF, ELISA Application Description |
| Cited Applications | WB, IP, IF, CoIP |
| Tested Reactivity | Recombinant protein |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | MYC-tag |
| Synonyms | |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881489 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| IF protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| IP protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia. | ||
Circ Res Yes-Associated Protein Promotes Angiogenesis Via Signal Transducer and Activator of Transcription 3 in Endothelial Cells. | ||
Nat Commun The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients. | ||
Cell Res ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer. |
