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MYL1 Polyclonal antibody

MYL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15814-1-AP

Print datasheet

Synonyms

MLC1/MLC3, MLC1F, MLC1F/MLC3F, MLC3F, MYL1, Myosin light chain A1/A2, Myosin light chain alkali 1/2

验证数据展示

  • WB analysis of human skeletal muscle using 15814-1-AP

    WB analysis of human skeletal muscle using 15814-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15814-1-AP (MYL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skeletal muscle using 15814-1-AP

    IHC staining of rat skeletal muscle using 15814-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL1 antibody included
  • IHC staining of rat skeletal muscle using 15814-1-AP

    IHC staining of rat skeletal muscle using 15814-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL1 antibody included
  • IHC staining of mouse skeletal muscle using 15814-1-AP

    IHC staining of mouse skeletal muscle using 15814-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL1 antibody included
  • IHC staining of rat skeletal muscle using 15814-1-AP

    IHC staining of rat skeletal muscle using 15814-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 15814-1-AP (MYL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL1 antibody included

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, human heart tissue, mouse heart tissue, mouse skeletal muscle tissue, mouse skin tissue
Positive IHC detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYL1 Ready-To-Use IHC Kit. MYL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15814-1-AP targets MYL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen MYL1 fusion protein Ag8594 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin, light chain 1, alkali; skeletal, fast
Synonyms MLC1/MLC3, MLC1F, MLC1F/MLC3F, MLC3F, MYL1, Myosin light chain A1/A2, Myosin light chain alkali 1/2
Calculated Molecular Weight150aa,17 kDa; 194aa,22 kDa
Observed Molecular Weight 14-17 kDa, 22-25 kDa
GenBank Accession NumberBC005318
Gene Symbol MYL1
Gene ID (NCBI) 4632
RRIDAB_2266747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05976
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYL1 antibody 15814-1-APDownload protocol
IHC protocol for MYL1 antibody 15814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
View Article
mouseWB

Cell Prolif

KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.

Authors - Jing Hao
View Article