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Mayer's 苏木素染色液

Cat no : PR30004

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Validation Data Gallery

  • FFPE human placenta tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

    FFPE human placenta tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

  • FFPE human ovary tumor tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

    FFPE human ovary tumor tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

  • FFPE human appendicitis tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

    FFPE human appendicitis tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

  • FFPE MCF-7 cell line was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

    FFPE MCF-7 cell line was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

  • FFPE mouse small intestine tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

    FFPE mouse small intestine tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

  • FFPE rat small intestine tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

    FFPE rat small intestine tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

  • FFPE rat tongue tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

    FFPE rat tongue tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

  • FFPE rat brain tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

    FFPE rat brain tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

  • Frozen mouse liver tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

    Frozen mouse liver tissue was stained with PR30004 (Mayer's Hematoxylin Solution) and PR30019 (Eosin Staining Solution).

  • Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody). PR30004 (Mayer's Hematoxylin) was used for counter staining

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody). PR30004 (Mayer's Hematoxylin) was used for counter staining

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66300-1-Ig (PR antibody) . PR30004 (Mayer's Hematoxylin) was used for counter staining.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66300-1-Ig (PR antibody) . PR30004 (Mayer's Hematoxylin) was used for counter staining.

  • The 10 mL size is provided in dropper bottles (left). 125 mL and 500 mL sizes are provided in narrow-neck bottles (right).

    The 10 mL size is provided in dropper bottles (left). 125 mL and 500 mL sizes are provided in narrow-neck bottles (right).

Product Information

Proteintech 生产的苏木素染色液适用于组织切片或培养细胞的染色，染色后细胞核呈蓝色。本产品经过改良处理，不含汞，甲醇等有毒试剂，使用简便，操作时间短。

本产品为即用型工作液，无需稀释。每毫升本产品大约可以染10张切片。

产品介绍

 组分规格

染色液

10 mL（滴瓶装)125 mL（窄口瓶装）500 mL（窄口瓶装）


保存条件

室温保存，至少一年有效。

使用方法

1. 免疫组化染色后的复染组织标本经过免疫组化显色后，将苏木素染液滴加在组织标本上，染色 1-2 分钟后，用蒸馏水将其从标本上冲洗干净，然后将标本浸入饱和 Na2HPO4 溶液中 2 分钟，待标本颜色变蓝后即可洗涤，脱水，透明，封片，观察。

2. HE 染色

 a.  对于石蜡切片，先在第一缸二甲苯中脱蜡 10 分钟，再在第二缸二甲苯中脱蜡 10 分钟，然后在各级酒精中分别浸泡 5 分钟，再用蒸馏水洗涤 2 分钟后，即可用苏木素染色，染色步骤同上。

 b.  对于冰冻切片，蒸馏水洗涤 2 分钟后，即可用苏木素染色，染色步骤同上。

 c.  对于培养细胞，先用 4%多聚甲醛固定 10 分钟以上，再用蒸馏水洗涤 2 分钟，然后换用新鲜的蒸馏 水，洗涤 2 分 钟后，即可用苏木素染色，染色步骤同上。

 d. 以上三种样品在用苏木素染色后，即可用伊红染色。

注意

1. 第一次使用本染色液时建议取 1-2 个样品做预实验优化至最佳条件。 

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

Publications

ApplicationTitle

Int J Nanomedicine

Therapeutic Efficacy of Carbon Ion Irradiation Enhanced by 11-MUA-Capped Gold Nanoparticles: An in vitro and in vivo Study

Authors - Pengcheng Zhang
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Free Radic Biol Med

PINK1/PARK2 dependent mitophagy effectively suppresses NLRP3 inflammasome to alleviate acute pancreatitis.

Authors - Jie Zhang
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IHC

Front Med (Lausanne)

Oridonin Alleviates LPS-Induced Depression by Inhibiting NLRP3 Inflammasome via Activation of Autophagy.

Authors - Chunyan Li
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Am J Physiol Heart Circ Physiol

Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.

Authors - Li Lin
View Article

Dis Markers

Targeting Circulating lncRNA ENST00000538705.1 Relieves Acute Coronary Syndrome via Modulating ALOX15.

Authors - Hao Chen
View Article

Evid Based Complement Alternat Med

Calycosin Improves Intestinal Mucosal Barrier Function after Gastrectomy in Rats through Alleviating Bacterial Translocation, Inflammation, and Oxidative Stress.

Authors - Hui Peng
View Article