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Mesothelin Monoclonal antibody, PBS Only

Mesothelin Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

2E3A4

Cat no : 66404-1-PBS

Print datasheet

Synonyms

MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor

验证数据展示

  • WB analysis of HeLa using 66404-1-Ig (same clone as 66404-1-PBS)

    WB analysis of HeLa using 66404-1-Ig (same clone as 66404-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • WB analysis using 66404-1-Ig (same clone as 66404-1-PBS)

    WB analysis using 66404-1-Ig (same clone as 66404-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

产品信息

66404-1-PBS targets Mesothelin in Indirect ELISA, IHC, WB applications and shows reactivity with human samples.

Tested Applications Indirect ELISA, IHC, WB Application Description
Tested Reactivity human
Immunogen Mesothelin fusion protein Ag23606 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mesothelin
Synonyms MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC003512
Gene Symbol MSLN
Gene ID (NCBI) 10232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13421
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).