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验证数据展示

  • WB analysis of HeLa using 66404-1-Ig (same clone as 66404-1-PBS)

    WB analysis of HeLa using 66404-1-Ig (same clone as 66404-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    IHC staining of human ovary tumor using 66404-1-Ig (same clone as 66404-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66404-1-Ig (Mesothelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

  • WB analysis using 66404-1-Ig (same clone as 66404-1-PBS)

    WB analysis using 66404-1-Ig (same clone as 66404-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66404-1-Ig (Mesothelin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66404-1-PBS in a different storage buffer formulation.

Mesothelin Monoclonal antibody, PBS Only

Mesothelin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66404-1-PBS

产品说明书

CloneNo. 2E3A4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66404-1-PBS targets Mesothelin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Mesothelin fusion protein Ag23606 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mesothelin
别名 MSLN, MPF Mesothelin, cleaved form, MPF, Mesothelin, cleaved form, Megakaryocyte-potentiating factor
计算分子量 69 kDa
观测分子量 69 kDa, 40 kDa
GenBank蛋白编号BC003512
基因名称 MSLN
Gene ID (NCBI) 10232
RRIDAB_2881778
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13421
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).