Mouse Adiponectin ELISA Kit
Validation Data Gallery
Product Information
KE10044 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous Adiponectin. The assay recognizes mouse Adiponectin. An antibody specific for Adiponectin has been pre-coated onto the microwells. The Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Adiponectin is added to detect the captured Adiponectin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Mouse Adiponectin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell Culture Supernates, Tissue homogenates, |
| Assay type | Sandwich |
| Sensitivity | 6.9 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11450 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 102% | 91%-122% |
| Mouse fat tissue Homogenates | 96% | 82%-106% |
| Cell Culture Supernates | 93% | 77%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 940.5 | 43.0 | 4.6 |
| 2 | 20 | 224.3 | 8.3 | 3.7 |
| 3 | 20 | 55.5 | 2.9 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,009.9 | 79.9 | 7.9 |
| 2 | 24 | 237.4 | 10.2 | 4.3 |
| 3 | 24 | 56.5 | 3.1 | 5.5 |
Background Information
Adiponectin, also known as Acrp30, AdipoQ, and GBP28, is an adipocyte-secreted protein that circulates in high concentrations in the serum and acts to increase insulin sensitivity. Adiponectin modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. The serum concentration of adiponectin decreases in obese patients, opposite to other adipocytokines. In recent studies, serum adiponectin level progressively decreased in patients according to the severity of obesity, insulin resistance, diabetes mellitus and cardiovascular diseases. Low serum adiponectin level may be a predictive factor for type 2 diabetes mellitus (T2DM) and other cardiovascular diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 30kDa, Acdc, Acrp30, adipo, Adipoq, apM1, APN, GBP28 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Cachexia Sarcopenia Muscle Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRα+ mesenchymal progenitors. | ||
Food Res Int Whey protein hydrolysate alleviated atherosclerosis and hepatic steatosis by regulating lipid metabolism in apoE-/- mice fed a Western diet. | ||
Hum Cell Enzyme-digested Colla Corii Asini (E'jiao) suppresses lipopolysaccharide-induced inflammatory changes in THP-1 macrophages and OP9 adipocytes. | ||
Diabetes Metab Syndr Obes Deep Sea Water Inhibited Pancreatic β-Cell Apoptosis and Regulated Glucose Homeostasis by Affecting Lipid Metabolism in Db/Db Mice | ||
Biochem Biophys Res Commun Types of cell culture inserts affect cell crosstalk between co-cultured macrophages and adipocytes | ||
Antioxidants (Basel) Transwell Culture with Adipose Tissue-Derived Stem Cells and Fertilized Eggs Mimics the In Vivo Development of Fertilized Eggs to Blastocysts in the Fallopian Tube: An Animal Study |