Mouse CCL22 ELISA Kit
Validation Data Gallery
Product Information
KE10053 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL22 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL22. The assay recognizes mouse CCL22. An antibody of biotinylated specific for CCL22 has been pre-coated onto the microwells. The CCL22 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL22 is added to detect the captured mouse CCL22 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CCL22 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.98 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20299 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 103% | 85%-121% |
| Cell culture supernatants | 101% | 83%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 251.2 | 6.7 | 2.7 |
| 2 | 20 | 56.3 | 2.5 | 4.5 |
| 3 | 20 | 12.2 | 1.2 | 9.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 215.9 | 20.9 | 9.7 |
| 2 | 24 | 55.7 | 3.4 | 6.0 |
| 3 | 24 | 12.7 | 1.3 | 9.9 |
Background Information
The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ccl22, DCBCK, MDC, Scya22 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Int Immunopharmacol CCL17 acts as an antitumor chemokine in micromilieu-driven immune skewing |