Mouse CXCL9/MIG ELISA Kit
Validation Data Gallery
Product Information
KE10067 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CXCL9/MIG ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CXCL9/MIG. The assay recognizes mouse CXCL9/MIG. An antibody specific for mouse CXCL9/MIG has been pre-coated onto the microwells. The mouse CXCL9/MIG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CXCL9/MIG is added to detect the captured mouse CXCL9/MIG protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CXCL9/MIG ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 0.48 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 17329 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 104% | 75%-124% |
| Tissue homogenates | 112% | 96%-120% |
| Cell culture supernates | 111% | 100%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 354.37 | 14.89 | 4.2 |
| 2 | 20 | 77.19 | 2.71 | 3.5 |
| 3 | 20 | 20.04 | 1.92 | 9.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 338.48 | 25.95 | 7.7 |
| 2 | 24 | 76.83 | 5.92 | 7.7 |
| 3 | 24 | 17.52 | 1.81 | 10.3 |
Background Information
CXCL9, also known as gamma-interferon induced monokine (MIG), is a member of the CXC family and plays an important role in the chemotaxis of the immune cells. It is produced by various immune and non-immune cells. CXCL9 can bind to the G protein-coupled receptor CXCR3 and recruit CXCR3 receptor expressed cells including activated T cells and NK cells. It has been shown to play a role in the immune checkpoint therapy. As a tumor suppressor, CXCL9 can recruit the tumor-infiltrating CD8+ T cells and NK cells, and CXCL9/CXCR3-B can inhibit the endothelial cell proliferation and tumor angiogenesis. As a tumor promoter, CXCL9/CXCR3-A can promote the tumor migration and invasion through the PI3K pathway, MAPK pathway, etc.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BB139920, CMK, crg 10, Cxcl9, Mig, Scyb9 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Biomaterials The programmed site-specific delivery of LY3200882 and PD-L1 siRNA boosts immunotherapy for triple-negative breast cancer by remodeling tumor microenvironment. | ||
Cancers (Basel) Nanosecond Pulsed Electric Field Induces an Antitumor Effect in Triple-Negative Breast Cancer via CXCL9 Axis Dependence in Mice | ||
Mol Ther Blockade of C5a receptor unleashes tumor-associated macrophage antitumor response and enhances CXCL9-dependent CD8+ T cell activity |