Mouse Cystatin C ELISA Kit
Validation Data Gallery
Product Information
KE10066 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse Cystatin C ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Cystatin C. The assay recognizes mouse Cystatin C. An antibody specific for mouse Cystatin C has been pre-coated onto the microwells. The mouse Cystatin C protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Cystatin C is added to detect the captured mouse Cystatin C protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Cystatin C ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Plasma, Serum, Cell culture supernates, Tissue homogenates, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.75 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13010 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 87% | 70%-122% |
| Cell culture supernates | 98% | 84%-108% |
| Tissue homogenates | 101% | 87%-115% |
| Urine | 96% | 75%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 504.29 | 19.54 | 3.9 |
| 2 | 20 | 119.07 | 4.99 | 4.2 |
| 3 | 20 | 30.11 | 1.89 | 6.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 512.12 | 14.02 | 2.7 |
| 2 | 24 | 122.52 | 6.91 | 5.6 |
| 3 | 24 | 33.43 | 1.68 | 5.0 |
Background Information
Cystatin C is a proteinase inhibitor from the cysteine protease inhibitor super family, which plays an important role in the intracellular catabolism of proteins and peptides. Cystatin C is produced by all the nucleated cells irrespective of age, gender, ethnicity, or muscle mass. The cystatin C based glomerular filtration rate (GFR) estimation can be used as a validation test for the poor prognosis of the chronic kidney disease patients. It has a pleiotropic effect in the human vascular pathophysiology that is related to the occurrence of coronary heart disease (CHD), ischemic stroke (IS), heart failure (HF), subclinical atherosclerosis and cardiovascular disease (CVD).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cst3, CysC, cystatin C |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Commun Non-canonical dihydrolipoyl transacetylase promotes chemotherapy resistance via mitochondrial tetrahydrofolate signaling | ||
bioRxiv A High-fat, High-salt Diet Model of MDAKD Impairs Bioenergetic Efficiency for ATP Synthesis. | ||
Hypertens Res Angiotensin II type 1 receptor-associated protein deletion combined with angiotensin II stimulation accelerates the development of diabetic kidney disease in mice on a C57BL/6 strain | ||
Eur J Pharmacol Topiroxostat improves glomerulosclerosis in type 2 diabetic Nagoya Shibata Yasuda mice with early nephropathy |
