Mouse ICAM-1/CD54 ELISA Kit
Validation Data Gallery
Product Information
KE10063 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse ICAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse ICAM-1. The assay recognizes mouse ICAM-1. An antibody specific for mouse ICAM-1 has been pre-coated onto the microwells. The mouse ICAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse ICAM-1 is added to detect the captured mouse ICAM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse ICAM-1/CD54 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 3.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 15894 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 94% | 82%-116% |
| Cell culture supernates | 89% | 78%-102% |
| Tissue homogenates | 86% | 79%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 443.5 | 10.3 | 2.3 |
| 2 | 20 | 107.1 | 2.4 | 2.2 |
| 3 | 20 | 23.6 | 0.6 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 463.4 | 7.4 | 1.6 |
| 2 | 24 | 108.6 | 2.7 | 2.5 |
| 3 | 24 | 24.0 | 0.6 | 2.7 |
Background Information
Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin. It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum. ICAM-1 can exist as membrane-bound form (mICAM-1) and soluble form (sICAM-1). The sICAM-1 arises from alternative splicing and proteolysis of mICAM-1 and appears to be associated with leukocyte activation to produce LFA-1.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD54, ICAM 1, Icam1, ICAM-1, Ly 47, MALA 2 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Biol Sci Olfactomedin-4 deletion exacerbates DSS-induced colitis through a matrix metalloproteinase-9-dependent mechanism | ||
J Cell Mol Med Intermittent hypoxia increased the expression of ESM1 and ICAM-1 in vascular endothelial cells via the downregulation of microRNA-181a1 | ||
Int J Biol Macromol Nattokinase attenuates endothelial inflammation through the activation of SRF and THBS1 |