Mouse/Rat IGF1 ELISA Kit
Validation Data Gallery
Product Information
KE10032 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat IGF1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat IGF1. The assay recognizes mouse/rat IGF1. An antibody specific for mouse/rat IGF1 has been pre-coated onto the microwells. The mouse/rat IGF1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat IGF1 is added to detect the captured mouse/rat IGF1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse/Rat IGF1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma, Rat serum, Rat plasma, Cell lysates, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16000 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 105% | 95%-119% |
| Rat serum | 103% | 79%-117% |
| Tissue lysates | 103% | 87%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 550.2 | 16.0 | 2.9 |
| 2 | 20 | 164.8 | 6.7 | 4.1 |
| 3 | 20 | 78.0 | 5.1 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 501.3 | 38.4 | 7.7 |
| 2 | 24 | 138.5 | 14.1 | 10.2 |
| 3 | 24 | 56.0 | 6.1 | 10.8 |
Background Information
Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone is the principal mediator of biochemical effects of growth hormone (GH). IGF1 is an important growth factor in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 have been shown to play an essential role in preventing the formation of fatty liver. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas. Mice lacking IGF1 exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C730016P09Rik, Igf 1, Igf I, Igf1, insulin like growth factor 1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) | ||
J Anim Sci Biotechnol Beneficial effects of a decreased meal frequency on nutrient utilization, secretion of luteinizing hormones and ovarian follicular development in gilts. | ||
iScience Exercise-acclimated microbiota improves skeletal muscle metabolism via circulating bile acid deconjugation | ||
Biochem Biophys Res Commun Lactococcus cremoris subsp. cremoris FC-fermented milk activates protein synthesis and increases skeletal muscle mass in middle-aged mice. | ||
Can J Physiol Pharmacol LPS-pretreated MSC-conditioned medium optimized with 10-kDa filter attenuates the injury of H9c2 cardiomyocytes in a model of hypoxia/reoxygenation. | ||
Biol Pharm Bull Juzentaihoto Suppresses Muscle Atrophy and Decreased Motor Function in SAMP8 Mice. |