Mouse IL-15 ELISA Kit
Validation Data Gallery
Product Information
KE10028 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-15 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-15. The assay recognizes mouse IL-15. An antibody specific for IL-15 has been pre-coated onto the microwells. The IL-15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-15 is added to detect the captured IL-15 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-15 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 50.2 pg/mL |
| Range | 250 -8000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16168 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 91% | 81%-97% |
| Cell culture supernatants | 110% | 99%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 323.3 | 23.3 | 7.2 |
| 2 | 20 | 837.1 | 52.1 | 6.2 |
| 3 | 20 | 3,300.7 | 215.3 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 242.7 | 21.0 | 8.6 |
| 2 | 24 | 868.7 | 68.0 | 7.8 |
| 3 | 24 | 3,355.6 | 201.9 | 6.0 |
Background Information
Interleukin 15 (Il15) is a cytokine important for the development, maturation, and function of many cells of the immune system including NK, NKT, gammadeltaT, and CD8(+) T cells. Its expression affects homeostasis and function of B cells through NK cell-derived IFNG. Il-15 binds a trimeric Il-15 receptor (Il-15R) complex formed by a specific receptor chain (Il-15Rα) combined with theβandγchains of the IL-2R, acting as signal transducing components. The transgenic mice overexpressing Il15 exhibit an increase in the number of memory CD8+ T cells in a naive state and enhanced protection against bacterial infections. Mice lacking Il15 exhibit impaired protection against a strain of attenuated Mycobacterium.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AI503618, Il15, IL-15, interleukin 15 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Mammary Gland Biol Neoplasia TGFß1 Stimulates Lymphatic Endothelial Cells to Produce IL7 and IL15, Which Act as Chemotactic Factors for Breast Cancer Cells with Mesenchymal Properties |