Mouse IL-2 ELISA Kit
Validation Data Gallery
Product Information
KE10004 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-2. The assay recognizes mouse IL-2. An antibody specific for IL-2 has been pre-coated onto the microwells. The IL-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-2 is added to detect the captured IL-2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16183 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 111% | 99%-120% |
| Cell culture supernatants | 107% | 94%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,176.7 | 74.6 | 6.3 |
| 2 | 20 | 358.5 | 17.4 | 4.8 |
| 3 | 20 | 64.1 | 3.5 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,090.8 | 88.5 | 8.1 |
| 2 | 24 | 331.5 | 28.7 | 8.7 |
| 3 | 24 | 58.3 | 2.9 | 4.9 |
Background Information
Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signal is mediated through heterodimerization of the IL2Rβ and Il-2Rγ receptor subunits.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il 2, Il2, IL-2, interleukin 2, interleukin-2 |
Publications
| Species | Sample Type | Title |
|---|---|---|
ACS Nano Self-Assembly of Podophyllotoxin-Loaded Lipid Bilayer Nanoparticles for Highly Effective Chemotherapy and Immunotherapy via Downregulation of Programmed Cell Death Ligand 1 Production. | ||
J Immunother Cancer ANKRD22 is a potential novel target for reversing the immunosuppressive effects of PMN-MDSCs in ovarian cancer | ||
Chin Med Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis | ||
Mol Pharm Cationic Nanoparticulate System for Codelivery of MicroRNA-424 and Podophyllotoxin as a Multimodal Anticancer Therapy. | ||
Inflammation Silencing TLR4/MyD88/NF-κB Signaling Pathway Alleviated Inflammation of Corneal Epithelial Cells Infected by ISE. | ||
Dermatol Ther Ameliorative effect of green odor against UVB-induced immunosuppression of contact hypersensitivity. |